First Minister Humza Yousaf must take SNP plans to use elections as de facto referendums off the table.
This comes after two senior SNP figures indicated that the party is still considering attempts to use that tactic at the next General Election.
Both depute leader Keith Brown and 'independence' minister Jamie Hepburn refused to rule out the proposals in recent days.
Pamela Nash, chief executive of Scotland in Union, said:
“As SNP leader and First Minister, Humza Yousaf must take these plans off the table once and for all.
“Even ardent independence supporters recognise trying to use an election as a de facto referendum is ludicrous.
“It’s hugely unpopular with the public, and has absolutely no reasonable basis for going ahead.
“Despite weeks of party crisis, coupled with the fact public services are on their knees, the SNP still seems determined to press ahead with this farcical proposal.
“But voters won’t tolerate it, because they want a government which is focused on things that really matter.
“It’s time for people’s priorities, not the SNP’s.”